Fluorescence Microscopy Biology Diagrams

Fluorescence Microscopy Biology Diagrams Fucci (Fluorescent Ubiquitination-based Cell Cycle Indicator)is a set of fluorescent probes which enable the visualization of cell cycle progression in living cells. Fucci takes advantage of the fact that the replication licensing factors Cdt1 and Geminin are only present during specific phases of the cell cycle. A fusion protein of a fragment

The expression levels of two closely linked proteins in the eukaryotic DNA replication network came in handy for Miyawaki's team when visualizing the cell cycle. Chromatin licensing and DNA replication factor 1 (Cdt1) "licenses" DNA to replicate during the cell cycle. Geminin inhibits Cdt1, preventing it from re-replicating DNA. A robust method for simultaneous visualization of all four cell cycle phases in living cells is highly desirable. We developed an intensiometric reporter of the transition from S to G2 phase and

Visualizing Spatiotemporal Dynamics of Multicellular Cell Biology Diagrams

Abstract. Proper cell-cycle progression is essential for the self-renewal and differentiation of human pluripotent stem cells (hPSCs). The fluorescent ubiquitination-based cell-cycle indicator (FUCCI) has allowed the dual-color visualization of the G 1 and S/G 2 /M phases in various dynamic models, but its application in hPSCs is not widely reported. In addition, lineage-specific FUCCI

Similarly to FUCCI, these proteins carry cell cycle-dependent degradation motifs to resolve G1 and S/G2/M phases. We showed utility of FUCCI-Red by visualizing cell cycle progression of cancer cells in 2D and 3D cultures and monitoring development of tumors in vivo by confocal and fluorescence lifetime imaging microscopy (FLIM).

color cell cycle indicator for fluorescence ... Biology Diagrams

By visualizing the cell cycle, Fucci is a powerful tool to investigate any process that has to do with cell growth and differentiation, such as the development and regeneration of organs as well as carcinogenesis. Cells with pure green fluorescence were either in the S or G2 phase and were distinguished by immunostaining of the S phase. The The widely used fluorescence ubiquitination cell cycle indicator (FUCCI) system, based on the expression of the cell cycle oscillators Cdt1 and geminin tagged with different fluorescent proteins The fluorescence ubiquitination cell-cycle indicator (FUCCI) was developed in 2008 by Miyawaki et al., which color-codes the phases of the cell cycle in real-time. Therefore, visualizing cell cycle dynamics within tumors with FUCCI can provide a guide for many strategies to improve cell-cycle targeting therapy for solid cancers. Keywords: